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Question #45119
The Question
Read the following statements regarding the biolistic (gene gun) method of direct gene transfer. Which of the following statements is INCORRECT?
Options
- A.
It utilizes high-velocity microparticles of heavy metals like gold or tungsten coated with recombinant DNA.
- B.
It is primarily suitable for plant cells because the microparticles possess enough kinetic energy to penetrate the rigid cellulosic cell wall.
- C.
Target plant cells with extensive lignified secondary walls exhibit higher transformation efficiency due to better retention of the microparticles.
- D.
Excessive kinetic energy required to breach highly thickened cell walls often leads to mechanical cell death rather than stable nuclear integration.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Comparative_Analysis
Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.
Concepts Behind This Question
Biolistics/Gene Gun Physics|Cry Gene Specificity
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students often assume that since the gene gun is specifically designed for 'rigid plant cell walls', a thicker or more rigid wall (like a lignified secondary wall) simply requires a 'stronger shot' without biological consequences.
The misconception lies in ignoring the delicate balance between particle penetration and cell viability.
High kinetic energy causes mechanical cell death (lysis), making highly lignified tissues extremely poor targets for biolistic transformation.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Biolistics/Gene Gun Physics |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |