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Question #46566

Difficulty: 8/10 | 🟢 Free

The Question

During industrial ethanol production, a cooling system failure causes the bioreactor temperature to exceed the thermal tolerance of the yeast, specifically denaturing the enzyme pyruvate decarboxylase. Which of the following represents the most immediate biochemical consequence of this thermal stress?

Options

  • A.

    Glycolysis arrests due to the inability to regenerate NAD+ from NADH, as the formation of the intermediate electron acceptor, acetaldehyde, is blocked.

  • B.

    Alcohol dehydrogenase directly utilizes the accumulated pyruvic acid as an electron acceptor to maintain the continuous regeneration of NAD+.

  • C.

    The metabolic flux shifts towards the mitochondrial electron transport chain to oxidize the accumulated NADH, independent of oxygen availability.

  • D.

    The yeast cells immediately switch to lactic acid fermentation, utilizing lactate dehydrogenase to sustain the optimal energetic yield of 2 ATP.

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Correct Answer

Step-by-Step Solution

Explanation

How the Examiner Thinks

Comparative_Analysis

Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.

Concepts Behind This Question

Saccharomyces_cerevisiae|Thermal Tolerance Ranges|Optimal Foraging Theory

Why This Question Is Tricky

Common Mistakes

Conceptual Pivot: Students often memorize the net equation of fermentation without understanding the sequential enzymatic dependency.

The core misconception is failing to recognize that the first enzyme (pyruvate decarboxylase) generates the specific electron acceptor (acetaldehyde) required by the second enzyme (alcohol dehydrogenase) to regenerate NAD+.

Quick Facts About This Question

Question Information

SubjectBiology
ChapterMicrobes in Human Welfare - Class 12
ConceptAlcoholic Beverage Fermentation Source
Difficulty8
Cognitive Load5
Target Solve Time120 sec
AccessFree