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Question #46728
The Question
During a biotechnology experiment, a researcher uses microinjection to introduce alien DNA directly into the nucleus of an animal cell. However, the cells consistently undergo cytoplasmic leakage and die immediately after the micropipette is withdrawn. Based on the principles of cellular anatomy, which of the following is the most likely underlying cause of this failure?
Options
- A.
The target cells possess a rigid cellulosic cell wall that shatters upon mechanical perforation.
- B.
A defect in the quasi-fluid nature of the lipid bilayer prevents the rapid lateral diffusion required for membrane resealing.
- C.
The host cell lacks the specific surface receptors required to recognize and internalize the recombinant DNA.
- D.
The procedure lacked the addition of divalent cations (like calcium) required to create transient pores for DNA entry.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Evolutionary_Exception
Tackle problems where standard rules evolve or change based on specific environmental constraints.
Concepts Behind This Question
Plasma_Membrane_Fluidity|Restriction_Enzyme_Cleavage|DNA_Replication
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Integrating Biotechnology (Microinjection) with Cell Biology (Fluid Mosaic Model).
Common Misconception: Students often memorize gene transfer methods in isolation.
This question tests the ability to differentiate the physical requirements of microinjection (intrinsic membrane fluidity for repair) from chemical competence (calcium treatment) and viral/receptor-mediated entry, while also recalling the basic structural differences between plant and animal cells.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Cell – The Unit of Life - Class 11 |
| Concept | Plasma Membrane Fluidity |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |