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Question #46944
The Question
During the optimization of a novel PCR protocol, a researcher isolates a hyperthermophilic Archaebacterium to extract a new thermostable DNA polymerase. However, standard cell lysis using lysozyme fails. Which of the following statements regarding this scenario and the organisms involved is INCORRECT?
Options
- A.
Lysozyme fails because the Archaebacterial cell wall lacks true peptidoglycan, containing pseudomurein or S-layer proteins instead.
- B.
Unlike the Archaebacterium, Thermus aquaticus, the standard source of Taq polymerase, is a Eubacterium with a typical peptidoglycan cell wall.
- C.
The extreme thermal stability of the Archaebacterium is solely attributed to the thermostability of its intracellular enzymes like DNA polymerase.
- D.
The cell membrane of the Archaebacterium contains branched-chain lipids, which decrease membrane fluidity and prevent disruption at high temperatures.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
Taq Polymerase Thermal Stability|Archaebacteria Cell Wall Composition|Extreme Thermophiles
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students frequently misclassify Thermus aquaticus as an Archaebacterium due to its thermophilic nature.
Additionally, because Biotechnology chapters focus heavily on the thermostability of the Taq polymerase enzyme, students develop a reductionist misconception that extreme heat tolerance is solely an enzymatic property, ignoring the NCERT Class 11 fact that the cell wall and branched membrane lipids are the primary structural adaptations for extreme survival.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biological Classification - Class 11 |
| Concept | Archaebacteria Cell Wall Composition |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |