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Question #47703
The Question
A geneticist is designing a somatic gene therapy protocol to treat a metabolic disorder. They utilize a viral vector and the restriction endonuclease HindIII to create a recombinant DNA molecule. Which of the following statements regarding the tools and principles used in this procedure is INCORRECT?
Options
- A.
If the vector is cleaved with HindIII and the therapeutic gene with EcoRI, the resulting sticky ends will lack complementarity, preventing successful ligation.
- B.
In the nomenclature of HindIII, the letter 'd' is derived from the name of the strain of the prokaryotic cell from which it was isolated.
- C.
The Roman numeral 'III' in HindIII indicates that it was the third restriction endonuclease isolated from the genus Haemophilus.
- D.
Because the therapy targets somatic cells, the successful integration of the therapeutic gene will not be transmitted to the patient's progeny.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Comparative_Analysis
Sharpen your ability to evaluate multiple scenarios and distinguish between competing potential answers.
Concepts Behind This Question
Restriction Endonuclease | DNA Methylation | Prokaryotic Defense
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: The exact definition of the Roman numeral in restriction enzyme nomenclature.
Students frequently memorize 'order of isolation' but miss the critical NCERT micro-detail: 'from that strain of bacteria'.
By substituting 'strain' with 'genus', Option C creates a high-fidelity 'Not/Except' trap that tests precise reading comprehension and taxonomic hierarchy.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Restriction Endonuclease Nomenclature |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |