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Question #47735
The Question
In recombinant DNA technology, Ethidium Bromide (EtBr) is routinely used to visualize DNA fragments. If a living, rapidly dividing cell culture is inadvertently exposed to EtBr, which of the following best describes the primary mechanism of its mutagenic effect during DNA replication?
Options
- A.
It covalently cross-links adjacent pyrimidines, leading to the stalling of DNA polymerase.
- B.
It acts as a structural analog of purines, causing transition mutations during elongation.
- C.
It inserts itself between adjacent base pairs, distorting the double helix and inducing frameshift mutations.
- D.
It binds specifically to the origin of replication (ori), preventing the assembly of the helicase enzyme.
Check Your Answer
Correct Answer
Step-by-Step Solution
Explanation
How the Examiner Thinks
Regulatory_Failure
Understand how NOT, EXCEPT, and logical constraints are used to manipulate exam results.
Concepts Behind This Question
LacZ_Gene|X-gal|Recombinant_Plasmid
Why This Question Is Tricky
Common Mistakes
Conceptual Pivot: Students heavily associate Ethidium Bromide strictly with 'orange bands under UV light' (Biotechnology chapter) and often miss its biochemical mechanism of action.
The trap tests the synthesis of Biotechnology (EtBr usage) with the Molecular Basis of Inheritance (types of mutations), specifically distinguishing intercalating agents (causing frameshifts) from base analogs or UV-induced damage.
Quick Facts About This Question
Question Information
| Subject | Biology |
| Chapter | Biotechnology : Principles and Processes - Class 12 |
| Concept | Gel Electrophoresis Migration |
| Difficulty | 8 |
| Cognitive Load | 5 |
| Target Solve Time | 120 sec |
| Access | Free |